The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay

Abstract Edible bird's nest (EBN) is a traditional food which was nourishing and functional. Particularly, there the epidermal growth factor (EGF) in EBN, thought to play an important role promoting skin repair. However, type content of EGF EBN were not determined yet. In this study, identified as bird by enzyme-linked immunosorbent assay method validated be accurate precise. Moreover, it found that raw-unclean raw-clean stewed 3000 pg/g–4000 pg/g no significant differences, suggested batches, origins, forms, stewing temperatures times had effect on EBN. due enzyme destroyed primary structure EGF, neutral protease trypsin hydrolysates lower than flavor enzymes, alkaline pepsin EGF. This study first determine provided theoretical basis for selection processing using source